. Author manuscript; available in PMC: 2020 Jan 6.

Published in final edited form as: Biochem J. 2014 Oct 1;463(1):145–155. doi: 10.1042/BJ20140522

Table 1. Substrate ranges of Arabidopsis TenA_E proteins and B. subtilis TenA_C.

Substrate concentrations were 1 mM. Activities were determined at 30°C in 45 mM Tris-HCl buffer, pH 7.5, containing 0.9 mM DTT and 0.45 M glycinebetaine. Results are means ± S.E.M. of six replicates.

Substrate	Specific activity (nmol min⁻¹ mg⁻¹)
Substrate	Arabidopsis TenA_E	B. subtilis TenA_C
Amino-HMP	0.34 ± 0.05	292 ± 34
Formylamino-HMP	6.52 ± 0.31	<0.05
Thiamin	<0.05	162 ± 18
Thiamin monophosphate	<0.05	<0.05
Thiamin diphosphate	<0.05	<0.05
Oxothiamin	<0.05	91 ± 20
Oxythiamin	<0.05	205 ± 30
Thiamin disulfide	<0.05	<0.05
Desthiothiamin	<0.05	<0.05
Thiochrome	<0.05	<0.05