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. Author manuscript; available in PMC: 2020 Jan 6.
Published in final edited form as: ACS Chem Biol. 2019 Feb 20;14(3):405–414. doi: 10.1021/acschembio.8b01038

Figure 5. MDP induces co-immunoprecipitation NOD2 and Arf6.

Figure 5.

(a) Western blot analysis of anti-myc immunoprecipitation of wild-type and Arf6 mutants in HA-NOD2-transfected HEK293T cells ± 1 μM MDP. (b) In-gel fluorescence analysis and click chemistry labeling with az-rhodamine of anti-HA immunoprecipitated HA-NOD2 from HEK293T cells co-transfected with Arf6-myc ± MDP (1 μM) or x-alk-MDP (20 μM) with UV-crosslinking. Western blot analysis of HA-NOD2 and Arf6 expression levels. (c) In-gel fluorescence analysis and click chemistry labeling with az-rhodamine of immunoprecipitated HA-NOD2 (wt and D379A mutant) from HEK293T cells co-transfected with wt Arf6-myc ± x-alk-MDP (20 μM) crosslinking.