Fig. 11.

Mtb-specific response in HIV-TB patients is modulated differentially by 7-OD and DHEA. Recently thawed or freshly isolated PBMCs from HIV-TB patients were stimulated with an antigen of Mtb in the presence/absence of 7-OD at 1 × 10⁻⁶M or DHEA at 1 × 10⁻⁷M. Then, cells were stained and analyzed by flow cytometry, as described before. This figure shows the percentage of CD4 + T cells that express (a) FoxP3, T-bet, ROR-γt or CD25 and the co-expression of transcription factors using a Boolean gating strategy or (b) CD4+ Tregs and uTregs, with the co-expression of the transcription factors ROR-γt or T-bet within each population. Values are relativized to unstimulated cells. The results are plotted for HIV-TB patients. Each symbol represents an individual subject. Friedman test followed by post-hoc comparisons: Fisher’s LSD or Dunn’s test, as appropriated *p < 0.05, **p < 0.01 and ***p < 0.005