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. 2020 Jan 6;27:20. doi: 10.1186/s12929-019-0604-z

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Analysis of Treg and uTreg subsets within CD4 + T cells from HIV-TB patients and HD. Recently thawed or freshly isolated PBMCs were stained and analyzed by flow cytometry. Figure shows the percentage of (a) CD4+ regulatory T cells (Tregs, CD25 + FoxP3+) and CD4+ unconventional regulatory T cells (uTregs, CD25-FoxP3+) and (b) the co-expression of the transcription factors ROR-γt or T-bet within each population. The results are plotted for HD (open circles) and HIV-TB patients (black circles). Each symbol represents an individual subject. Unpaired t test or Mann-Whitney U test, as appropriated *p < 0.05. Representative flow cytometry examples are shown