Fig. 9.

Mtb induces changes in CD4+ T-cell phenotype from HIV-TB patients. Recently thawed or freshly isolated PBMCs from HIV-TB individuals were stimulated with an antigen of Mtb, stained and analyzed by flow cytometry, as indicated in methods. Figure shows the percentage of CD4 + T cells that express (a) FoxP3, T-bet, ROR-γt or CD25 and (b) the co-expression of transcription factors using a Boolean gating strategy. c Tregs (CD4+ CD25+ FoxP3+) and uTregs (CD4+ CD25- FoxP3+) subsets were also analyzed. Representative flow cytometry examples are shown. Each symbol represents an individual subject. Unpaired t test or Mann-Whitney U test, as appropriated *p < 0.05, **p < 0.01 and ***p < 0.005