Fig. 3.

LNCs reduce protein synthesis in breast cancer cells. Protein synthesis was measured in a MCF-7 cells, b MDA-MB-231 cells and c MDA-MB-468 cells. The cells were treated with 0.5 mg/ml of LNCs for the indicated times at 37 °C in culture medium supplemented with 1% or 10% FCS. Protein synthesis was measured by incorporation of [³H]leucine for the final 10 min. Data are shown as mean ± SEM from three independent experiments; *p < 0.05; **p < 0.01. d Recovery of protein synthesis after removal of LNCs from the medium. MCF-7 cells were treated for 4 h at 37 °C with 0.5 mg/ml LNC; the cell medium was removed, and the cells washed with PBS before fresh medium was added and incubation continued for 0.5, 1.0 and 20 h. The experiment with the longest incubation time was performed with 10% FCS only, as the cells did not tolerate to be incubated that long with only 1% FCS. The data are shown as percent of untreated controls as the mean ± SEM from three independent experiments; ^#p < 0.05; ^##p < 0.01; ^###p < 0.001 for comparison with cells incubated with LNCs for 4 h