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. 2020 Jan 6;27:21. doi: 10.1186/s12929-019-0606-x

Fig. 1.

Fig. 1

Melatonin regulates the level of Muc2 in intestinal epithelial cells treated with rVvpM. Dose (a) and time (b) responses of rVvpM in Mucin (Muc)2 mRNAs of HT29-MTX cells are shown. Data represent means ± S.E. n = 5. *, p < 0.05 vs. 0 pg/mL. c Cells were treated with melatonin at various concentrations (10 nM ~ 1 μM) for 30 min prior to rVvpM (100 pg/mL) exposure for 12 h. Expression of Muc2 is shown. Data represent means ± S.E. n = 4. *, p < 0.05 vs. vehicle. #, p < 0.01 vs. rVvpM alone. d Time responses of 100 pg/mL of rVvpM in Muc2 ELISA assay are shown. Error bars represent the means ± S.E. n = 4. *, p < 0.01 vs. 0 h. HT29-MTX (e) and Caco-2 human intestinal epithelial cells (f) were incubated with melatonin (1 μM) for 30 min prior to rVvpM exposure for 24 h. The level of Muc2 production was quantified by ELISA. Data represent means ± S.E. n = 4. *, p < 0.01 vs. vehicle. #, p < 0.05 vs. rVvpM alone