Fig. 2.

Induction of pig operon at 37°C enables prodigiosin production.

A. Pigment production at 30°C in liquid culture after 18 h of growth at 30°C. The wild-type strain was modified by recombination of pMQ262 through which an L-arabinose-inducible promoter replaces the pig promoter. B. Induced expression of pig at 37°C enabled expression of prodigiosin demonstrates that prodigiosin can be made at 37°C when the pig operon is expressed using pMQ262. Images show representative macrocolonies (see materials and methods).