Table I.
Strains and plasmids used in this study.
Strain | Description | Source |
---|---|---|
PIC3611 | Wild-type Serratia marcescens | Presque Isle Cultures |
CMS1687 | PIC3611 crp-Δ4 mutant | (Kalivoda et al. 2010) |
CMS2097 | PIC3611 eepR mutant | (Stella et al. 2015) |
CMS2922 | PIC3611 hexS mutant | (Shanks et al. 2013) |
CMS4891 | PIC3611 pigB::tn-lux pigment operon reporter | this study |
CMS4892 | PIC3611 pigF::tn-lux pigment operon reporter | this study |
Plasmid | Relevant information | Source |
pMQ99 | Plac-hcred shuttle vector | this study |
pMQ132 | pBBR1-based shuttle vector | (Shanks et al. 2009) |
pMQ200 | oriR6K-based suicide plasmid with PBAD | (Shanks et al. 2009) |
pMQ221 | pMQ132 with pigP gene | (Shanks et al. 2013) |
pMQ262 | pMQ200 with pigAB’ | (Kadouri and Shanks, 2013) |
pMQ364 | pMQ132 with eepR gene | (Stella et al. 2015) |
pMQ690 | promoterless-luxCDABE transposon delivery plasmid | this study |
pMQ713 | pig promoter-luxCDABE transcriptional fusion | this study |