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. 2019 Jan 1;12(1):164–172.

Figure 3.

Figure 3

MiR-381 directly inhibits ROCK2 by targeting its 3’UTR. A. Predicted binding sequences of miR-381 in the 3’UTR of ROCK2. B. The ROCK2 3’UTR containing wild-type (WT) or mutant (Mut) 3’UTR of ROCK2 was subcloned into the luciferase reporter vector, then luciferase assay was performed in BGC823 cells cotransfected with miR-381 mimic and a luciferase reporter containing the wild-type (WT) or mutant (Mut) 3’UTR of ROCK2. Luciferase activity was determined 48 h after transfection. C. The expression levels of ROCK2 protein in BGC823 cells after 48 h transfection with miR-381 mimic, miR-381 inhibitor or scramble mimic (NC) were measured western blot. *P<0.05 versus the negative control (NC), data shown are means ± S.D.