Figure 3.

(A) Agarose gel electrophoresis analysis of CPL and EGF-CPL combined with DNA at various weight ratios. Lane 1: DNA control; lanes 2-14: NPs/DNA weight ratios of 1:1 to 13:1. In vitro luciferase expression (B) and EGFP expression of fluorescent intensity (C) in SKOV3 cells. Different wells of cells were transfected with naked plasmid, lipo 2000/DNA complexes, CPL/DNA complexes and EGF-CPL/DNA complexes at varied weight ratios of total CPLs/DNA of 3/1, 6/1 and 9/1 at a dose of 1 μg of DNA per well. The EGFP plasmid was used; at 48 h, fluorescent microscopy was performed.