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Decreased HCN2 channel expression inhibited the activation of astrocytes and microglia in ipsilateral spinal dorsal horns. Representative immunofluorescent staining of GFAP (A) and IBA1 (B) to detect activated astrocytes and microglia, respectively. Treatment with ZD-7288 and si-HCN2 obviously inhibited the activation of astrocytes and microglia in L4-L5 ipsilateral spinal dorsal horns.
