Figure 2.

Cell apoptosis mediated by ROS was attenuated and tumorigenesis was promoted by miR-17-5p. (A-D). NPC cells were transfected with miR-17-5p mimics or a negative control (NC) and followed by 10 Gy irradiation. TUNEL (A, B) and Annexin V/PI (C, D) staining were performed to detect the apoptotic cells (n = 5). (E, F) The NPC cells were treated as (A) and the non-irradiation groups as controls. The ROS generation was analyzed with a mean fluorescence index by flow cytometry (n = 5). (G-I) The NPC cells were stably transfected with miR-17-5p or a negative control (NC) and inoculated subcutaneously into the back of mice followed by irradiation treatment. The tumors’ volume (H) and weight (I) were determined (n = 3). *: P < 0.05, **: P < 0.01.