Figure 4.

Mir-17-5p’s effect on NPC cell radioresistance was retrieved by the AKT signaling blockade. A. CNE2 cells overexpressed miR-17-5p in the presence of the AKT inhibitor were detected in the cell cycle using flow cytometry (n = 5). B. The number of colonies were counted in the CNE2 cells with same treatment as above (n = 5). C. CNE2 cells with same treatment as above were cultured in 96-well plates and the growth curve was measured (n = 5). D. The apoptosis of CNE2 cells treated as above was detected using TUNEL staining (n = 5). E. The apoptosis of the CNE2 cells treated as above was determined with Annexin V/PI staining followed by flow cytometry analysis (n = 5). F. The intracellular ROS generation was detected by flow cytometry and quantified by a mean fluorescence index (n = 5). Note: 1: NC; 2: mimics; 3: mimics + AKT inhibitor. G. The schematic diagram of miR-17-5p protecting the NPC cells from irradiation damage. *: P < 0.05, **: P < 0.01.