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. 2019 Jan 1;12(1):50–63.

Figure 3.

Figure 3

miR-214-3p is a target of HOTAIR in HCC (A) The binding sequence of the HOTAIR 3’UTR with miR-214-3p. (B) The expression of miR-214-3p was significantly decreased in HCC tissues and their adjacent normal tissues by qRT-PCR. (C) The expression of miR-214-3p was evidently enhanced in Hep3B, HepG2 cells. (D) miR-214-3p expression was significantly increased in miR-214-3p group compared with miR-NC group and obviously decreased in miR-214-3p inhibitor group compared with inhibitor-NC. (E and F) The luciferase activities were measured in Hep3B and HepG2 co-transfected with a luciferase reporter containing miR-214-3p mimics, HOTAIR WT and HOTAIR MUT. (G and H) The luciferase activities were measured in Hep3B and HepG2 co-transfected with a luciferase reporter containing miR-214-3p inhibitor, HOTAIR WT, and HOTAIR MUT. (I) HOTAIR expression was markedly up-regulated in Hep3B and HepG2 with transfection of pcDNA-HOTAIR. (J) The expression level of miR-214-3p after transfection of pcDNA, pcDNA-HOTAIR, siNC and siHOTAIR in Hep3B and HepG2. (K) miR-214-3p expression was negatively correlated with the expression of HOTAIR. *P < 0.05.