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NOTCH1 knock-down arrests cell cycle at the G0/G1 phase. Cell cycle was determined by propidium iodide (PI) staining followed by flow cytometric analysis. (A–D) The cell cycles of AGS control cells (Vec) and cells with NOTCH1 knock-down (shN1-1, shN1-2, shN1-3) were tested by flow cytometry analysis. (E) Cell cycle distributions of AGS cells with or without NOTCH1 knock-down. * P<0.05.
