Figure 6:

Human cell lines expressing HER2 mutants are also sensitive to poziotinib. (A-C) Dose response curves of MCF10A cells expressing HER2 exon 20 insertion mutants G776delinsVC (A), Y772dupYVMA (B), or G778dupGSP (C), treated with indicated TKIs for 72 hr. (D) Dot plot of ratio of IC₅₀ values comparing MCF10A expressing HER2 exon 20 mutants to MCF10A expressing WT HER2. Dots are representative of mean ± SEM for each cell line and bars are representative of mean ± SEM of all three cell lines (n ≥ 3 for each cell line). (E) Dose response curve of CW-2 colorectal cancer cell line harboring endogenous ERBB2 exon 19 mutation, L755S, treated with indicated inhibitors for 72 hr. (A-C, E) Curves are representative of mean ± SEM, n = 3. (F) Bar graph of CW-2 tumor volume at day 21. Tumors were randomized at 350 mm³, indicated by the dotted line. Dots are representative of individual tumors (n = 5/ group), and bars are representative of mean ± SEM. See also Figure S5.