KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
HER2	Cell Signaling Technologies	https://www.cellsignal.com/products/primary-antibodies/her2-erbb2-d8f12-xp-rabbit-mab/4290?site-search-type=Products
HER2	Abcam	https://www.abcam.com/erbb-2-antibody-ep1045y-ab134182.html
p-HER2 Tyr1248	Cell Signaling Technologies	https://www.cellsignal.com/products/primary-antibodies/phospho-her2-erbb2-tyr1248-antibody/2247?site-search-type=Products
pHER2 Tyr1221/1222	Cell Signaling Technologies	https://www.cellsignal.com/products/primary-antibodies/phospho-her2-erbb2-tyr1221-1222-6b12-rabbit-mab/2243?site-search-type=Products
pAkt	Cell Signaling Technologies	https://www.cellsignal.com/products/primary-antibodies/phospho-akt-ser473-d9e-xp-rabbit-mab/4060?site-search-type=Products
Akt	Cell Signaling Technologies	https://www.cellsignal.com/products/primary-antibodies/akt-antibody/9272?site-search-type=Products
p-p44/42	Cell Signaling Technologies	https://www.cellsignal.com/products/primary-antibodies/phospho-p44-42-mapk-erk1-2-thr202-tyr204-d13-14-4e-xp-rabbit-mab/4370?site-search-type=Products
P44/42	Cell Signaling Technologies	https://www.cellsignal.com/products/primary-antibodies/p44-42-mapk-erk1-2-137f5-rabbit-mab/4695?site-search-type=Products
Ubquitin	Cell Signaling Technologies	https://www.cellsignal.com/products/primary-antibodies/ubiquitin-antibody/3933?site-search-type=Products
tubulin	Cell Signaling Technologies	https://www.cellsignal.com/products/primary-antibodies/b-tubulin-antibody/2146?site-search-type=Products
Rabbit IgG	Santa Cruz	https://datasheets.scbt.com/sc-2027.pdf
Goat Anti-Rabbit IgG (H+L) HRP Conjugate	BioRad	http://www.bio-rad.com/en-us/sku/1706515-goat-anti-rabbit-igg-h-l-hrp-conjugate?ID=1706515
Chemicals, Peptides, and Recombinant Proteins
Erlotinib	Selleck Chem	https://www.selleckchem.com/products/erlotinib.html
Lapatinib	Selleck Chem	https://www.selleckchem.com/products/lapatinib.html
Afatinib	Selleck Chem	https://www.selleckchem.com/products/BIBW2992.html
Dacomitinib	Selleck Chem	https://www.selleckchem.com/products/pf299804.html
Neratinib	Selleck Chem	https://www.selleckchem.com/products/Neratinib(HKI-272).html
Poziotinib (in vitro / in vivo)	Selleck Chem	https://www.selleckchem.com/products/poziotinib-hm781-36b.html
Osimertinib	Selleck Chem	https://www.selleckchem.com/products/azd9291.html
Ibrutinib	Selleck Chem	https://www.selleckchem.com/products/pci-32765.html
Nazartinib	Selleck Chem	https://www.selleckchem.com/products/nazartinib-egf816-nvs-816.html
Olmutinib	Selleck Chem	https://www.selleckchem.com/products/olmutinib-hm61713-bi-1482694.html
Rocelitinib	Selleck Chem	https://www.selleckchem.com/products/co-1686.html
Tarloxotinib-TKI	Synthesized by IACS
T-DM1	MD Anderson institutional pharmacy
Poziotinib (clinical trial)	Spectrum Pharmaceuticals
Critical Commercial Assays
Cell Titer Glo	Promega	https://www.promega.com/products/cell-health-assays/cell-viability-and-cytotoxicity-assays/celltiter_glo-luminescent-cell-viability-assay/?catNum=G7570
ECL	Advansta	https://advansta.com/products/western-blot-substrate-WesternBright-ECL/
Crosslink Immunoprecipitation	Pierce	https://www.thermofisher.com/order/catalog/product/26147
HER2 ELISA	Cell signaling	https://www.cellsignal.com/products/elisa-kits/total-her2-erbb2-sandwich-elisa-kit/7310
Pan-pHER2 ELISA	Cell Signaling	https://www.cellsignal.com/products/elisa-kits/phospho-her2-erbb2-pantyr-sandwich-elisa-kit/7968?_=1563400997725&Ntt=pHER2%20ELISA&tahead=true
Experimental Models: Cell Lines
Ba/F3	Creative Biogene	https://www.creative-biogene.com/support/ba-f3-cell-line.html
MCF10A	ATCC	https://www.atcc.org/products/all/CRL-10317.aspx
CW-2	Riken Cell Bank	http://cellbank.brc.riken.jp/cell_bank/CellInfo/?cellNo=RCB0778
Experimental Models: Organisms/Strains
HER2 Y772dupYVMA PDX	JAX Labs	http://tumor.informatics.jax.org/mtbwi/pdxDetails.do?modelID=TM01446
Athymic Nude Mice	Harlan Envigo	https://www.envigo.com/products-services/research-models-services/models/research-models/mice/mutant/athymic-nude-mice/
NSG Mice	JAX Labs	https://www.jax.org/strain/005557
Recombinant DNA
ERBB2 L755S c.2264T>C	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 D769H c.2305G>A	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 D769N c.2305G>C	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 D769Y c.2305G>T	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 Y772dupYVMA c.2323_2324insTATGTCATGGCT	Purchased from Addgene (#40982)
ERBB2 G776delinsVC c.2326_2328insTCT	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 G776del insVV c.2327delinsTTGT	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 G776del insLC c.2326G>TTGT	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 V773M c.2317G>A	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 V777L c.2329G>T	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 G778insLPS c.2332_2333insGGCTCCCCA	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 P780insGSP c.2339_2340insTGGCTCCCC	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 L786V c. 2356C>G	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 V842I c.2524G>A	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
ERBB2 L869R c.2606T>G	Created by Bioinnovatise from pBabe-puro ERBB2 WT from Addgene (#40978)
Other
HER2 Crystal Structure	PDB	https://www.rcsb.org/structure/3PP0