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. 2019 Dec 5;14(1):230–231. doi: 10.1002/1878-0261.12604

Figure 4.

Figure 4

Deletion of RICTOR exon 5 disrupts the interaction between RICTOR and mTOR. (A) Schematic illustrating design of single‐guide RNA and primers for CRISPR/Cas9‐mediated deletion of exon 5 of RICTOR. (B) Predicted genotypes and base pair sizes for genomic PCR amplicons of RICTOR following CRISPR/Cas9 targeting of RICTOR. (C) Agarose gel images showing RICTOR amplicons in cell populations (FaDu, Cal27 cells) transfected with guides targeting RICTOR or an empty vector (PX458‐CMV). (D) Immunoblot of RICTOR expression in parental and mutant cell lines (E5‐XX lines). (E) Immunoblot showing co‐IP of RICTOR and mTOR in FaDu and Cal27 cells, but no detectable interaction in any of the putative RICTOR knockout cell lines.