Figure 6.

RSK1 signature can infer RSK1 levels from GBM transcriptome data. (A) The 33 genes of the RSK1 signature. Genes for which the RSK1 signature is shared by the mesenchymal subtype signature from TCGA (2010 and 2016) and/or LM22 signatures are indicated. (B) Relationship between GSVA scores for the RSK1 signature and patient survival. Colors indicate whether the samples belong to RSK1^hi or RSK1^lo groups defined in Fig. 2A. The vertical line indicates the separation of GBMs with GSVA score > −0.05 (signRSK1^enriched) and < −0.05 (signRSK1^{underrepresented}), and the horizontal line indicates the longest survival time for RSK1^hi group. The P‐value of the Fisher’s exact test was calculated based on the four groups generated by the vertical and horizontal lines and is indicated. (C) Overall survival plot comparing signRSK1^enriched and signRSK1^{underrepresented} groups. The number of samples is indicated in parentheses. (D) RSK1 protein (HSCORE) and (E) RSK1 mRNA (microarray) levels correlate with GSVA scores for RSK1 signature in the 30 GBM originally used to obtain the signature (ACCCC). (F, G) The RSK1 signature correlates with RSK1 mRNA levels from two public cohorts: (F) Gravendeel and (G) TCGA. The dashed line indicates the GSVA score cutoff for the survival curve in H and I. (H,I) Overall survival plots for (H) Gravendeel and (I) TCGA. The number of samples is indicated in parentheses. (J,K) Relationship between GSVA scores for the RSK1 signature and patient survival in (J) Gravendeel and (K) TCGA cohorts. The vertical line indicates the cutoff for signRSK1^enriched and signRSK1^{underrepresented} groups, and the horizontal line indicates the cutoff for long and short survival times. The P‐value of the Fisher’s exact test was calculated based on the four groups generated by the vertical and horizontal lines and is indicated along with the number of samples in each quadrant (censored samples below the survival time cutoff were not included). Status: 1 = dead; 0 = censored.