Figure 1. N metabolism gene expression patterns using RT-qPCR analysis for resistant (I59) (A, C, E and G) and susceptible (CV99) (B, D, F and H) genotypes under N sufficiency at 0, 12, 24 and 48 h post-inoculation with mock and rust inoculated coffee leaves.

The vertical bars indicate the values presented by the mean ± standard error (n = 9) of N metabolism gene for each time post-inoculation and genotype. Averages followed by the same capital letter, compare differences between mock and rust inoculated coffee leaves at the same HPI, did not differ significantly by the Tukey test (p < 0.05). Averages followed by the same lowercase letter, compare differences between the treatments (mock and inoc) at different HPI, did not differ significantly by the Tukey test (p < 0.05). CaGAPDH was used as reference gene and 0 HPI mock inoculation as calibrator.