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. 2019 Dec 26;25:10008–10015. doi: 10.12659/MSM.918172

Figure 4.

Figure 4

The inhibition of miR-506 repressed LPS-induced inflammation in DPSCs. DPSCs were transfected with miR-506 inhibitor or NC inhibitor for 24 h followed by LPS treatment for 8 h. (A) Q-PCR was used to examine miR-506 levels in cell lysates. (B) ELISA was conducted to assess IL-1β, IL-6, and TNF-α expression. (C, D) Cell lysates were subjected to Q-PCR to assess TLR4 and NFκB p65 expression. (E) WB analysis was performed to probe with antibodies for phosphorylated p65, total p65, and TLR4 in cell lysates. Data are represented as means±SD. * P<0.05; ** P<0.01.