FIGURE 3.

(A) Representative small-animal PET/CT images of mouse hind limbs 40 min after ¹⁸F-FDG injection (5–10 MBq/50 μL, intravenously) and 27 h after first succinate (right hind limb) or fumarate or PBS (left hind limb) injection every 6 h for 24 h, along with graph showing quantifications in each hind limb expressed as percentage injected dose per gram of tissue (%ID/g) over time from dynamic small-animal PET/CT reconstruction. *P < 0.05, Mann–Whitney test, 3 mice per condition. (B) Representative small-animal PET/CT images of mouse hind limbs 40 min after ¹⁸F-fluorocholine injection (5–7 MBq/50 μL, intravenously) and 27 h after first succinate (right hind limb) or fumarate or PBS (left hind limb) injection every 6 h for 24 h, along with graph showing quantifications in each hind limb expressed as %ID/g over time from dynamic small-animal PET/CT reconstruction. P = 0.617 vs. PBS and P = 0.923 vs. fumarate, respectively, not statistically significant, Mann–Whitney test, 3 mice per condition. (C) At top are representative laser-Doppler perfusion images of hind limbs from 6 mice immediately after ¹⁸F-FDG PET (28 h after first succinate [right hind limb] or fumarate or PBS [left hind limb] injection every 6 h for 24 h). At bottom are corresponding quantifications of perfusion signal in each hind limb.