. Author manuscript; available in PMC: 2021 Jan 2.

Published in final edited form as: Chemistry. 2019 Dec 3;26(1):249–258. doi: 10.1002/chem.201903808

Table 5.

EPR parameters obtained from simulation of EPR spectra of Cu(II)-protein solutions.

		g_x	g_y	g_z	A_∥ (10⁻⁴ cm⁻¹)	f=g_z/ A_∥ (cm)
^[a] GRα₃D H₃	75% A	2.05	2.04	2.27	166	136.8

	25% B	2.05	2.03	2.22	149	149.0

^[a] GRα₃D H₄	50% A	2.012	2.04	2.26	158	143.0

	50% B	2.04	2.03	2.23	188	119.6

^[a] GRα₃D H₂DH	N/A	2.06	2.03	2.27	162	140.2

^[a] GRα₃D H₃D	N/A	2.04	2.04	2.26	168	134.5

^[b] Cu-only SOD	N/A	2.05	2.05	2.26	∼140	161.4
^[c] Cu-(Fe) SOD	N/A	2.05	2.05	2.37	136	174.3

^[a]

X-band EPR spectra recorded at 100 K on solutions of 1 mM CuCl₂ and 2 mM protein in 50 mM HEPES buffer containing 30% glycerol at pH 7.5, 9.3 GHz, 20.5 mW, 1 G.

^[b]

Spagnolo et al., J. Biol. Chem., 2004, 33447–33455. X-band EPR spectra of a Cu-only SOD from Mycobacterium tuberculosis

^[c]

Kardinahl et al., Biol. Chem., 2000, 381, 1089–1101. X-band EPR spectra recorded at 10 K of a copper-substituted FeSOD from the archaeon Acidianus ambivalens in Tris/HCl buffer at pH 7, 9.6452 GHz, 20 mW, and 10.0 G.