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. Author manuscript; available in PMC: 2020 Oct 17.
Published in final edited form as: J Phys Chem B. 2019 Oct 3;123(41):8662–8674. doi: 10.1021/acs.jpcb.9b07228

Figure 2.

Figure 2.

a) Schematic for labeling of SLO mutants Q322C and S596C with Badan. b) Structure of SLO (PDB: 3PZW) labeled with Badan at the tip of the 317–334 surface loop (red) or the tip of the 586–603 surface loop (blue). Badan attached to the Cys-modified locations is depicted in green, and its orientation is arbitrary, for illustration purposes. The proposed thermally activated network is depicted in magenta (encircled), and the active site iron is shown as an orange sphere.