Figure 1.

PCB153 exposure regime and the effects on survival. A. Zebrafish embryos were exposed to either control or 3 different concentrations of PCB153 (0.1, 1, and 10 µM) starting from 4 h post-fertilization (hpf) until 5 days post-fertilization (dpf). Water was changed daily and fresh PCB153 was added (red arrows). At the end of the exposure, larvae were sampled for RNA seq. A subset of embryos were raised for phenotypic observation and behavioral assessment at 7 and 14 dpf. B. Percent survival in PCB153 exposed zebrafish embryos. No significant mortalities were observed during the 5-day exposure period. Mortalities were first observed at 7–8 dpf; up to 80% of the 10 µM treated group died by 14 dpf. No significant mortalities were observed in 0.1 and 1 µM PCB153-treated groups in comparison to dimethyl sulfoxide controls (n = 4 independent experiments).