Fig. 1.

Schematic of the steps commonly involved in co-elution methods. A, General workflow of a co-elution experiment. The lysed sample containing protein complexes under native conditions is separated in a set number of fractions. Proteins from the same complex show the same co-elution profile after a bioinformatic analysis to extract an interactome map. B, Co-elution data representative from an experiment where lysed HeLa cells were fractionated by size exclusion chromatography (SEC), quantified by MS/MS, and finally used to construct an interactome network through data analysis.