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. Author manuscript; available in PMC: 2020 Jan 6.
Published in final edited form as: Sci Transl Med. 2017 Nov 22;9(417):eaag1209. doi: 10.1126/scitranslmed.aag1209

Fig. 4. Restriction of TCR antigen to hematopoietic tissues does not prevent CAR8 exhaustion and failure of leukemia clearance.

Fig. 4.

(A) Female/male hematopoietic chimeras were generated by CD3-depleted bone marrow transplantation 21 days before injection of 1 × 106 E2aPbx followed by lymphodepletion on day −1 and treatment with 1 × 106 CD19 CAR T cells or mock-transduced T cells on day 0. (B and C) Survival of HY-CAR8–or HY-CAR4–treated mice. (D and E) Leukemia burden in the bone marrow 7 days after infusion of CAR T cells. (F and G) PD-1 expression and (H and I) LAG3 expression on HY-CAR8 and HY-CAR4 7 days after infusion [for (B) to (I): *P < 0.05, ****P < 0.0001 (relative to the F→F group), one-way ANOVA performed for all comparisons and log-rank Mantel-Cox test for survival analysis].