Figure 7.
vglu-2 expression in AIA is required for normal induction of reversals in response to diacetyl removal. (A) Animals freely moving in microfluidic devices were exposed to various diacetyl concentrations (lower-right panel, t < 0). The cumulative probability of reversal initiation in response to diacetyl removal (t > 0) was quantified (mean + SD). (B) Change in cumulative probability of initiating reversals within 5 sec after 1 µM diacetyl removal relative to the buffer–buffer transition probability (control). Significance was calculated by bootstrap with false discovery rate correction for multiple hypotheses (genotypes). *** P < 5 × 10−5. Similar results were obtained using the Kolmogorov–Smirnov test (Materials and Methods). Shown statistics: median (horizontal lines), 25th to 75th percentiles (boxes), and 5th to 95th percentiles (vertical lines). Number of animals (repeats): N2, 226 (678); Pgcy-28d::unc-103(gf), 44 (132); vglu-2(gk625231), 60 (180); vglu-2(gk625231;Pmgl-1::vglu-2 (OH16032), and 46 (138); vglu-2(gk625231);Pmgl-1::vglu-2 (OH16031), 45 (135). See Figure S4 for full results. ns, not significant; WT, wild-type.