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. 2019 Nov 18;214(1):147–161. doi: 10.1534/genetics.119.302772

Figure 6.

Figure 6

puf-3 puf-11 lie parallel to fbf-1 fbf-2 in the GSC regulatory pathway. (A) Results of epistasis tests conducted with lst-1(gf) and sygl-1(gf) alleles at 25°. Number of germ cells (GCs) in Glp animals was determined by counting sperm in adults and dividing by four. Genotype for lst-1(gf) strain is lst-1(ok814); qSi267 [Pmex-5::LST-1::3xFLAG::tbb-2 3′ end] fbf-1(ok91) fbf-2(q704) and sygl-1(gf) is sygl-1(tm5040); qSi235 [Pmex-5::SYGL-1::3xFLAG::tbb-2 3′ end] fbf-1(ok91) fbf-2(q704). We used the puf-3/11 RNAi clone B in these experiments. Images of representative germlines are available in Figure S5, A–D. (B) Results of epistasis tests conducted with gld-1 gld-2 at 20°. Genotype is gld-2(q497) gld-1(q361); fbf-1(ok91) fbf-2(q704). We used the puf-3/11 RNAi clone B in these experiments. Images of representative germlines are available in Figure S5, E and F. (C) Revised pathway for GSC regulation that includes puf-3 and puf-11 at the same position in the pathway as fbf-1 and fbf-2 (blue shading).