(
A,B) Number of AGO targets (
A) and miRNA targets (
B) analyzed in (
C–H) and in
Figure 5C,D. (
C–E) AGO targets are enriched in PBs (
C), translationally derepressed (
D) and little or not stabilized (
E) following DDX6 silencing. Two-tail Mann-Whitney test was performed with respect to all mRNAs. **, p<0.0001; *, p=0.002, x, p=0.04; ns, non-significant. (
F) AGO targets excluded from PBs accumulate following DDX6 silencing like other PB-excluded transcripts. The analysis was performed as in
Figure 5—figure supplement 2C. Two-tail Mann-Whitney test was performed with respect to cognate PB-out or PB-in mRNAs. x, p=0.04; ns, non-significant. (
G) AGO targets accumulate following PAT1B silencing. Two-tail Mann-Whitney test was performed with respect to all mRNAs. **, p<0.0001; x, p=0.04. (
H) The extent of PB enrichment of miRNA targets depends on the miRNA. Two-tail Mann-Whitney test was performed with respect to all mRNAs. **, p<0.0001; ns, non-significant.