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. 2019 Dec 31;10(68):7220–7237. doi: 10.18632/oncotarget.27322

Figure 8. SD-101 and low-dose CY combination drives increased activity of CD8+ T cells, which are required for efficacy of treatment.

Figure 8

(A) Frequency of intratumoral Tregs in response to indicated treatments, n=8-16/group. (B) Ratio of CD8+ T cells to Tregs in the tdLN and tumor. (C) Frequency of CD8+ T cells and CD4+ Tconv (CD3+ CD4+ FOXP3-) in the tdLN. (A–C) Tumors and tdLN collected following 5 doses of i.p. CY and 4 doses of i.t. SD-101, given twice weekly. (D) Expression of costimulatory molecules on APCs in the tdLN. CD40 (MFI) was measured on CD24+ MHCII+ cells that were CD3-CD19-SiglecF-Ly6G-. CD80 (MFI) was measured on CD11c+ MHCII+ cells that were CD3-CD19-SiglecF-Ly6G-. Tumor draining LN collected 2 days following the last dose of 2 doses of i.p. CY given twice weekly and 1 dose of i.t. SD-101 (approx. d19). (E) Tumor volume resulting from depletion of CD8+ T cells during combination treatment. Data are the mean ± SEM, n=10/group. * indicates P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001. (A–E) Representative graphs of at least two independent experiments.