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. 2019 Dec 31;10(68):7198–7219. doi: 10.18632/oncotarget.27319

Figure 2. Propionibacterium freudenreichii WT strain modulates in vitro expression of in Toll-like receptors (TLRs) in HT-29 cells .

Figure 2

Relative expression of genes encoding TLR2 (A), TLR4 (B), TLR9 (C), ZO1 (D) and of MUC2 (E), in HT-29 cells stimulated by lipopolysaccharides (LPS), P. freudenreichii 129 WT, P. freudenreichii 129ΔslpB, or combinations thereof, was monitored by RT-PCR. Each cell treatment was done on 3 independent cultures (biological triplicates). Each quantification was done in triplicate (technical triplicates). The means and standard deviations are thus calculated from 9 values. Asterisks represent statistically significant differences between strains and were indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001, and ****p < 0.0001.