Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) |
Rosa26-LSL-Cas9 knockin | Jackson Laboratory | IMSR Cat# JAX:026175, RRID:IMSR_JAX:026175 | |
| Genetic reagent (M. musculus) |
H11-CAG-Cas9 knockin | Jackson Laboratory | IMSR Cat# JAX:028239, RRID:IMSR_JAX:028239 | |
| Cell line (H. sapiens) | HEK293T | American Type Culture Collection | ATCC Cat# CRL-3216, RRID:CVCL_0063 | |
| Antibody | rat monoclonal anti-HA | Roche | Roche Cat# 3F10, RRID:AB_2314622 | ICC 1:1000 |
| Antibody | mouse monoclonal anti-alpha-Dystroglycan | Millipore | Millipore Cat# 05–593, RRID:AB_309828 | ICC 1:200 |
| Antibody | mouse monoclonal anti-HA | Covance | Cat# AFC-101P-1000, RRID:AB_291231 | WB 1:1000 |
| Antibody | rabbit polyclonal anti-GFP | Molecular Probes | Cat# A-11122, RRID:AB_221569 | WB 1:1000 |
| Antibody | mouse monoclonal anti-Gephyrin | Synaptic Systems | Cat# 147 011, RRID:AB_887717 | ICC 1:300 |
| Antibody | rabbit polyclonal anti-MAP2 | Synaptic Systems | Cat# 188 002, RRID:AB_2138183 | ICC 1:1000 |
| Antibody | mouse monoclonal anti-beta-Tubulin III | Sigma-Aldrich | Cat# T8660, RRID:AB_477590 | ICC 1:1000 |
| Antibody | rabbit polyclonal anti-GABAARα1 | Synaptic Systems | Cat# 224 203, RRID:AB_223218 | ICC 1:500 |
| Antibody | rabbit polyclonal anti-GABAARα2 | Synaptic Systems | Cat# 224 103, RRID:AB_2108839 | ICC 1:500 |
| Antibody | rabbit polyclonal anti-GABAARβ3 | Synaptic Systems | Cat# 224 403, RRID:AB_2619935 | ICC 1:500 |
| Antibody | rabbit polyclonal anti-GABAARγ4 | Synaptic Systems | Cat# 224 003, RRID:AB_2263066 | ICC 1:500 |
| Antibody | rabbit polyclonal anti-Vgat | Synaptic Systems | Cat# 131 002, RRID:AB_887871 | ICC 1:1000 |
| Antibody | mouse monoclonal anti-dystrophin | Abcam | Cat# ab7164, RRID:AB_305740 | ICC 1:100 |
| Antibody | rabbit polyclonal anti-c-Fos | Millipore | Cat# ABE457, RRID:AB_2631318 | IHC 1:5000 |
| Recombinant DNA reagent | pSpCas9(BB)−2A-GFP (PX458) | Addgene | RRID:Addgene_48138 | |
| Recombinant DNA reagent | pBetaActin-HA-α 1-syntrophin | This paper | See Materials and methods section | |
| Recombinant DNA reagent | pBetaActin-HA-β-dystrobrevin | This paper | See Materials and methods section | |
| Recombinant DNA reagent | pAAV-U6-sgRNA-hSyn-Cre | PMID: 27609886 | backbone of AAV CRISPR constructs | |
| Recombinant DNA reagent | pAAV-U6-InSyn1Cterm-HITI-smFP-SynI-Cre | This paper | See Materials and methods section | |
| Recombinant DNA reagent | pAAV-mTubb3 | Addgene | RRID:Addgene_87116 | HITI construct |
| Recombinant DNA reagent | pAAV-hSyn-hChR2(H134R)-EYFP | Addgene | RRID:Addgene_26973 | |
| Sequenced-based reagent | gRNAs | This paper | See Materials and methods section | |
| Chemical compound, drug | InFusion cloning kit | TaKaRa | Cat#638910 | |
| Chemical compound, drug | Bis(2,2,2-trifluoroethyl) ether/Flurothyl | Santa Cruz Bioteh | 333-36-8 | |
| Software, algorithm | GraphPad Prism | GraphPad Prism (https://graphpad.com) | RRID:SCR_002798 | Version 8 |
| Software, algorithm | SPSS | IBM | RRID:SCR_002865 | |
| Software, algorithm | ImageJ | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_001935 | 1.52e |
| Software, algorithm | Puncta Analyzer/ImageJ | PMID: 21113117 | 1.29 |