FIG 6.

Role of SREBP1 isoforms in TLR4-dependent regulation of fatty acid synthesis. BMDM from C57BL/6J, SREBP1a-deficient (SREBP1a-DF), and SREBP1c knockout (SREBP1c-KO) mice were treated with the vehicle, 100 ng/ml KLA, or 1.0 μM GW3965 for 16 h. Following treatment, mRNA levels of Srebf1a (A), Srebf1c (B), Fads2 (C), Scd1 (D), Scd2 (E), and Lxrα (F) were quantified by real-time PCR and normalized to the value for cyclophilin. *, statistically significant difference from the vehicle control within the same genotype; **, statistically significant difference between C57BL/6 and Srebp1 knockouts determined by 2-way ANOVA (P ≤ 0.05; data derived from 2 mice/genotype and 4 replicates/mouse).