FIG 9.

Increased ISG expression in LXR knockout macrophages. (A) Heat map of RNA-Seq data (n = 3/group) comparing 38 ISGs in KLA-treated C57BL/6 and Lxrα^−/−/Lxrβ^−/− BMDM. (B to D) C57BL/6 and Lxrα^−/−/Lxrβ^−/− BMDM were treated with the vehicle, 100 ng/ml KLA, or 1.0 μM GW3965 for 16 h. Following treatment, mRNA levels of Gbp9 (B), Irf1 (C), and Stat1 (D) were quantified by real-time PCR and normalized to the value for cyclophilin. Statistically significant differences between C57BL/6 and Lxrα^−/−/Lxrβ^−/− mice determined by 2-way ANOVA (P ≤ 0.05; n = 10) are noted. Data are means ± standard errors.