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. Author manuscript; available in PMC: 2020 Jun 17.
Published in final edited form as: Sci Signal. 2019 Dec 17;12(612):eaaw9252. doi: 10.1126/scisignal.aaw9252

Fig. 2. KIR2DL1 allotypes differ in their nanoscale organization.

Fig. 2

(A) Flow cytometry analysis of representative pNK cell clones from a single donor that expressed different KIR2DL1 allotypes, stained for KIR2DL1 (gray) or with an isotype-matched control (white). (B) Representative TIRF microscopy and Gaussian-rendered STORM images of pNK cell clones with different KIR2DL1 allotypes (scale bar: 3 μm). The 2 × 2 μm regions (red boxes in the STORM images) are magnified and the corresponding scatter plots, density maps, and binary maps are shown (scale bar: 1 μm). The Ripley’s H function is plotted for a 3 × 3 μm region containing the enlarged region. (C) Quantitative analysis of the binary maps. Each dot represents the mean value for a cell. Red bars represent the median and IQR. Mann Whitney test: KIR2DL1*004 (13 cells, 1 clone, 1 donor), KIR2DL1*001 (11 cells, 3 clones, 1 donor). **P < 0.01. See also fig. S4 for analogous data with KIR3DL1 and KIR2DL3.