Biopsies in the future liver remnant in a liver without alterations, before (A) and after (B) Tourniquet ALPPS (H&E, 100×). The normal architecture of the liver is preserved in both and there are no significant differences between them. (C) Second stage biopsy of cirrhotic liver (Masson’s Trichrome, 40×) after Tourniquet ALPPS. In contrast to the previous images, the normal architecture of the liver is replaced by multiple regeneration nodes separated from each other by fibrous septa, and no relevant differences can be highlighted when compared with a cirrhotic liver previous to the surgery (D, H&E 40×).