Figure 2.

Gal-1 negatively controls the differentiation of imDCs into mature DCs by targeting NF-κB/RelB (n=3). After transfection, cells were incubated in 4 conditions as indicated for 24 h more and then assayed by flow cytometry. (A) CD80; (B) CD45RB; (C) CD11c; (D) MHC II expression and population were qualified. Cytokines such as (E) IL-27 and (F) TGF-β were measured by ELISA. Values indicate mean ± SEM of these experiments. *, P<0.05; **, P<0.01. imDCs, immature dendritic cells; NC, negative control; Gal, galectin; MHC, major histocompatibility complex; DC, dendritic cell; ELISA, enzyme-linked immunosorbent assay; SEM, standard error of mean.