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. 2019 Nov;7(22):649. doi: 10.21037/atm.2019.10.85

Figure 4.

Figure 4

miRNA-142-3p suppresses IFN-γ expression and did so by stimulating RICTOR/AKT signaling AFE activated CD4+ T cells were transfected with control siRNA or RICTOR siRNAs for 24 h. The efficacy of RICTOR knockdown was measured by qPCR (A) and Western blotting (B) analysis; (C) p-473 AKT was detected by Western blotting; (D) the qPCR assay was implemented to measure IFN-γ expression in AFE activated CD4+ T cells that were transfected with control siRNA or RICTOR siRNAs. **, P<0.001 as compared with control. miRNA, microRNAs; AFE, Aspergillus fumigatus.