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. 2019 Nov;7(22):643. doi: 10.21037/atm.2019.10.71

Figure 3.

Figure 3

miR-3619-3p activates Wnt/β-catenin pathway in PTC. (A) The relative expression level of CTNNB1(β-catenin) was significantly up-regulated in PTC tissues compared with corresponding adjacent non-tumor tissues by qRT-PCR (n=36); (B) the association analysis of the relationship between miR-3619-3p and CTNNB1 expression levels in 36 paired PTC tissues; (C) the relative mRNA expression level of CTNNB1 in BCPAP and TPC-1 after treatment by qRT-PCR; (D) immunoblotting detected protein levels of β-catenin in BCPAP and TPC-1 that were transiently transfected with NC, miR-3619-3p mimics or inhibitor; (E) the CTNNB1 RNA stability assay in TPC cells with treatment of 10 ng/mL actinomycin D after miR-3619-3p mimics or NC transfection. The data represent the mean ± SD from three independent experiments. *, P<0.05; **, P<0.01. PTC, papillary thyroid carcinoma.