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. 2019 Nov;7(22):643. doi: 10.21037/atm.2019.10.71

Figure 4.

Figure 4

miR-3619-3p enhances cell migration and invasion via activating Wnt/β-catenin pathway in PTC. (A,B) Transwell migration and invasion assays for BCPAP that were transfected with miR-3619-3p mimics or NC followed by treatment with ICG-001 (10 µm) for 24 h. (C,D) Transwell migration and invasion assays for BCPAP that were transfected with miR-3619-3p inhibitor or NC after treatment with LiCL (20 mM) for 24 h. (A,C) The cells were stained with 0.05% crystal violet. Representative images of migration and invasion are shown; all images were obtained at 100× magnification under an inverted microscope. The cells that had migrated were counted from ten randomly chosen fields. (E,F,G,H) Wound healing assays of BCPAP after treatment, respectively. **, P<0.01. Error bars correspond to the mean ± SD of triplicate experiments. PTC, papillary thyroid carcinoma.