Effects of GLI1 on the expression of MMP2 and MMP9. (A) Human endometrial stromal cells were treated with GANT-61 (15 µM), ad/sh-Scramble or ad/sh-GLI1 1, and then the expression of MMP2 and MMP9 mRNA were measured by quantitative real-time PCR; (B) representative Western blots of GLI1, MMP2 and MMP9 protein from human endometrial stromal cells which were treated with GANT-61 (15 µM), ad/sh-Scramble or ad/sh-GLI1 1; (C) the quantitative comparison of the fold difference in the expression of GLI1, MMP2 and MMP9 protein. The data are presented as the means ± SD of three independent experiments (*, P<0.05; **, P<0.01 by one-way ANOVA).