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. 2019 Nov;7(22):634. doi: 10.21037/atm.2019.10.108

Figure 4.

Figure 4

Gal improves myocardial I/R injury by activating AMPK/Nrf2 pathway. (A) Rats were grouped into five groups (10 in each group): Sham group, I/R model, I/R + Gal (1 mg/kg), I/R + Gal (3 mg/kg), and I/R + Aspirin (20 mg/kg) group. The protein levels of AMPKα1, Nrf2, and HO-1 were measured by western blotting assay. Actin was used as an internal reference. *, P<0.05 vs. sham group; #, P<0.05 vs. I/R group. (B,C,D,E,F) Rats were grouped into five groups (10 in each group): Sham group, I/R model, I/R + Gal (3 mg/kg), I/R + CC (200 µM), and I/R + Gal (3 mg/kg) + CC (200 µM) group. (B) The protein levels of AMPKα1, Nrf2, and HO-1 were measured by western blotting assay. (C) LVESV and FS. (D) The protein levels of CHOP and BiP were measured by western blotting assay. (E) The mRNA level of Caspase 3 was measured by RT-qPCR. (F) The protein levels of α-SMA and collagen I were measured by western blotting assay. Actin was used as an internal reference. *, P<0.05 vs. sham group; #, P<0.05 vs. I/R group; &, P<0.05 vs. I/R + CC group.