Figure 3.

Effects of single treatments or osimertinib plus DHA combination on proteins involved in the downstream signaling of the EGFR pathway and RTKs. Western blot analysis showing protein levels in FaDu and CAL27 cells treated with DHA (A) and osimertinib plus DHA (B). In A, cells were treated with increasing doses of DHA for 24 hours. In B, cells were treated with the single drugs (0.5 µM osimertinib, and 20 µM DHA) or the combination and protein extracts were taken at 24 h after treatment. B-actin was used as loading control. Data were generated from a minimum of three replicates. DHA, dihydroartemisinin; EGFR, epidermal growth factor receptor; RTK, receptor tyrosine kinase.