Table 1.
Cost of genome sequencing for a cancer case (tumor and germline sample)
| Stage | Cost category | Total | % Total test costs before overheads | ||
|---|---|---|---|---|---|
| Equipment | Consumables | Staff | |||
| Sample reception | £0.24 | £0.31 | £17.59 | £18.14 | 0.3% |
| DNA extraction | £0.19 | £20.91 | £15.95 | £37.06 | 0.7% |
| Nanodrop | £0.16 | £0.11 | £6.20 | £6.47 | 0.1% |
| Qubit | £0.19 | £3.05 | £11.20 | £14.43 | 0.3% |
| Agarose gel | £0.15 | £14.26 | £22.70 | £37.11 | 0.7% |
| Library processinga | £51.83 | £132.09 | £84.64 | £268.56 | 4.7% |
| Sequencingb | £615.24 | £3688.04 | £48.55 | £4351.83 | 76.3% |
| Bioinformatics | £1.80 | £266.73 | £406.99 | £675.52 | 11.8% |
| Reporting | £0.00 | £0.00 | £257.25 | £257.25 | 4.5% |
| Data archiving | £24.56 | £0.96 | £8.83 | £34.35 | 0.6% |
| Total (before overheads) | £694.35 | £4126.46 | £879.89 | £5700.71 | - |
| % total cost | 12% | 72% | 15% | - | - |
| Total (including overheads calculated at 20%) | - | - | - | £6840.85 (£3420.43 per genome) | - |
aLibrary processing includes library preparation, normalization, and validation.
bSequencing also includes clustering.