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. 2019 Jul 24;22(1):150–159. doi: 10.1038/s41436-019-0606-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Expression of DHX37 in mouse and human developing testis. (a–c) Expression in embryonic mouse gonads at sex-determining stage of 11.5 dpc (17–21 tail somites [ts]). (a) Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis of Dhx37 in wild-type (WT) mouse gonads reveals no significant difference in expression between XX and XY (p = 0.056, Student’s t test). Expression is relative to Hrpt1; error bars represent standard error. (b) Whole-mount in situ hybridization reveals Dhx37 expression in WT XY gonad (to the left of the dashed line). Signal appears stronger towards the coelomic surface. (c) Immunofluorescence of transverse sections of WT XY gonad (left of the dashed line) showing DHX37 (green), OCT4 (red, primordial germ cells), and DAPI (blue). DHX37 protein is concentrated around the nuclear membrane in most cells, although this is not the case in germ cells (arrows). Cells of the mesonephros (right of the dashed lines) are also positive for Dhx37/DHX37 (b, c). (d) DHX37 protein examined in human fetal testis. No protein is observed in gonocytes (marked with arrows), while protein is found in Sertoli cells (marked with asterisk) and in a subpopulation of the interstitial cells. Sample is from a gestational week (GW) 10 fetus. Counterstaining with Mayer’s hematoxylin, scale bar corresponds to 50 µm.