Skip to main content
. 2020 Jan 2;40(1):BSR20193158. doi: 10.1042/BSR20193158

Figure 4. RHOA is a direct target of miR-154-3p and miR-487-3p.

Figure 4

Using on-line bioinformatics algorithm (TargetScan, http://www.targetscan.org/), the conserved sequence of binding sites between miR-154-3p/-487-3p and RHOA was shown across various species (A); miR-154-3p and miR-487-3p are identified as candidate miRNAs targeting to RHOA (B). The association between miR-154-3p/487-3p and RHOA is further examined by evaluating luciferase activity in human thyroid cancer cell lines K-1 and B-CPAP (C). After transfection with pre-miR-Con, pre-miR-154-3p, pre-miR-487-3p or pre-miR-154-3p/487-3p into K-1 and B-CPAP cells, the mRNA and protein levels of RHOA are detected using RT-PCR and Western blotting, respectively (D); * P < 0.05; ** P < 0.01.