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. 2020 Jan 1;7:8. doi: 10.1038/s41438-019-0223-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Phenotypic characterizations of wild-type and mutant cotyledons. Plants 20 DAS (days after sowing). Bars = 3 cm. b Phenotypic characterizations of wild-type and nym1 rosette leaves. Plants were at the field-grown heading stage (50 DAS). Bars = 10 cm. c Pigment content of wild type and mutant bottom leaves. Plants 60 DAS. Ten plants from the wild type and “nym1” lines with the same growth potential were randomly selected. The second leaves from the bottom were sampled. **Significantly different at P = 0.01 by the t test. d, e Transmission electron microscopy (TEM) of WT and mutant bottom leaves. The plant stage was the same as that for Fig. 1c. Three leaf specimens were sampled per plant. Fifteen sections were viewed by TEM. Twelve cells were examined and photographed per section. S starch granule, OG and arrow osmiophilic plastoglobuli, G granum. Bars = 5 μm. f Phenotypic characterizations of wild type and mutant leafy heads. Plants 70 DAS. Left panel: wild type; right panel: nym1. Bars = 7 cm.