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. 2020 Jan 6;6:1. doi: 10.1038/s41523-019-0144-4

Fig. 2. In vitro validation and functional screening of lncRNA candidates.

Fig. 2

a RNA expression obtained by qRT-PCR for four of the lncRNA candidates in a panel of cell lines. MCF7 cell line was used as reference. b Subcellular localization of the lncRNA candidates assessed by qRT-PCR after nuclear–cytoplasm fractionation. c Viability assays (MTT) after knock-down (siRNA 20 nM or LNA 50 nM) in two different cell lines for each of the lncRNA candidates at different time points. d RNA levels of the lncRNA candidates after knock-down in two cell lines at 48 and 72 h after transfection. e Migration and invasion in the MDA-MB-468 cell line after lncRNA AC01719.1 knock-down. Experiments were performed at least three times in triplicate. Data are presented as mean ± SEM. *P < 0.05; **p < 0.01; ***p < 0.001 (two-tailed unpaired t test).